A benchmark of 13 data transformations and normalizations commonly used in microbiome research, using simulated quantitative datasets. Methods were evaluated to assess their performance in recovering richness and diversity from the simulated data, and in their determining microbe-microbe and microbe-metadata associations.

The simulated data as well as the code to reproduce the results of the publication is available at: https://github.com/raeslab/benchmark_microbiome_transformations